Journal: Discover Oncology

Article Title: Novel N 6 -methyladenosine (m 6 A) writer METTL16 promotes the cervical cancer tumorigenesis by targeting FTH1-dependent ferroptosis

doi: 10.1007/s12672-026-04403-8

Figure Lengend Snippet: FTH1 acted as the target of METTL16. A The immunofluorescence assay showed the subcellular localization of METTL16 and FTH1. B The binding motif sites of METTL16 for its target genes (GGAAC). C The FTH1 level in cervical cancer cells was test by the RT-PCR. D RNA immunoprecipitation (RIP) assay was conducted for the binding within METTL16 and FTH1 in HeLa cells. E RIP assay using anti-m 6 A conducted for the m 6 A modification on FTH1 mRNA. * p < 0.05, ** p < 0.01

Article Snippet: Cervical cancer cells were lysed by immunoprecipitation buffer (protease, phosphatase inhibitors, MCE) containing magnetic beads conjugated to specific antibodies (anti-IGF2BP2, anti-METTL16, anti-m 6 A).

Techniques: Immunofluorescence, Binding Assay, Reverse Transcription Polymerase Chain Reaction, RNA Immunoprecipitation, Modification

Journal: Discover Oncology

Article Title: Novel N 6 -methyladenosine (m 6 A) writer METTL16 promotes the cervical cancer tumorigenesis by targeting FTH1-dependent ferroptosis

doi: 10.1007/s12672-026-04403-8

Figure Lengend Snippet: IGF2BP2 promoted the stability of FTH1 mRNA. A The immunofluorescent staining showed the subcellular localization of IGF2BP2 and FTH1 in HeLa cells. B The positive correlation within IGF2BP2 and FTH1 in clinical samples ( http://gepia.cancer-pku.cn/index.html ). C RNA immunoprecipitation (RIP) assay showed the immunoprecipitated FTH1 mRNA by anti-IGF2BP2 antibody. D RNA decay analysis reported the stability of FTH1 mRNA in cervical cancer cells with IGF2BP2 overexpression. E RNA decay analysis reported the stability of FTH1 mRNA in cervical cancer cells with METTL16 silencing (sh-METTL16-1, sh-METTL16-2). F RNA decay analysis reported the stability of FTH1 mRNA in cervical cancer cells with IGF2BP2 silencing (si-IGF2BP2) and METTL16 overexpression. * p < 0.05, ** p < 0.01

Article Snippet: Cervical cancer cells were lysed by immunoprecipitation buffer (protease, phosphatase inhibitors, MCE) containing magnetic beads conjugated to specific antibodies (anti-IGF2BP2, anti-METTL16, anti-m 6 A).

Techniques: Staining, RNA Immunoprecipitation, Immunoprecipitation, Over Expression

Journal: Experimental and Therapeutic Medicine

Article Title: Insulin-like growth factor 2 promotes the adipogenesis of hemangioma-derived stem cells

doi: 10.3892/etm.2018.7132

Figure Lengend Snippet: IGF-2 stimulated the proliferation of HemSCs in vitro . (A) IGF-2 promoted the proliferation of HemSCs in vitro ; IGF-2 at 100 and 200 ng/ml significantly promoted the proliferation of HemSCs as compared with the control group, and IGF-2 at 100 ng/ml exhibited the highest proliferation activity among all groups. (B) Growth curves of IGF-2 treated HemSCs. The proliferation of IGF-2-treated HemSCs (days 1–7) was promoted compared with the untreated group. Values are mean ± SD, n =3; *P<0.05 and **P<0.01 vs. Control group; # P<0.05 vs. 100 ng/ml. IGF-2, insulin-like growth factor-2; HemSC, hemangioma stem cell.

Article Snippet: The cells were cultured in EGM-2/FBS-10% containing IGF-2 (100 ng/ml), IGF-2 (100 ng/ml) plus OSI-906 (1 μM), IGF-2 (100 ng/ml) plus LY294002 (cat. no. HY-10108; 10 μM; MedChem Express Co., Ltd., Shanghai, China), or no treatment.

Techniques: In Vitro, Control, Activity Assay

Journal: Experimental and Therapeutic Medicine

Article Title: Insulin-like growth factor 2 promotes the adipogenesis of hemangioma-derived stem cells

doi: 10.3892/etm.2018.7132

Figure Lengend Snippet: Expressions of IGF-2 and its receptors in HemSCs and the effect of IGF-2 on the adipogenesis of HemSCs. (A) Immunohistochemistry of HemSCs using antibodies specific to IGF-2, IGF-2R, IGF-1R, and IR. (B) IGF-2 induced differentiation of HemSCs into adipocytes. HemSCs that were cultivated for 10 days in adipogenic differentiation media; adipogenic differentiation media with IGF-2 (100 ng/ml); adipogenic differentiation media with IGF-2 (100 ng/ml) plus OSI-906 (1 µM). After Oil Red O-staining, phase-contrast microscopy was used to observe lipid droplets. Scale bar, 100 µm. IGF-2, insulin-like growth factor-2; HemSC, hemangioma stem cell; IGF-1R, IGF-1 receptor; IR, insulin receptor.

Article Snippet: The cells were cultured in EGM-2/FBS-10% containing IGF-2 (100 ng/ml), IGF-2 (100 ng/ml) plus OSI-906 (1 μM), IGF-2 (100 ng/ml) plus LY294002 (cat. no. HY-10108; 10 μM; MedChem Express Co., Ltd., Shanghai, China), or no treatment.

Techniques: Immunohistochemistry, Staining, Microscopy

Journal: Experimental and Therapeutic Medicine

Article Title: Insulin-like growth factor 2 promotes the adipogenesis of hemangioma-derived stem cells

doi: 10.3892/etm.2018.7132

Figure Lengend Snippet: Effects of IGF-2 on the expression of adipogenic markers in HemSCs. The C/EBPα, C/EBPβ, PPAR, and adiponectin protein levels were analyzed by western blot analysis. (A) The results showing protein levels in untreated and treated HemSCs for C/EBPα, C/EBPβ, PPARγ, and adiponectin in IGF-2 (100 ng/ml), IGF-2 (100 ng/ml) with OSI-906 (1 µM), IGF-2 (100 ng/ml) with LY294002 (10 µM), and LY294002 (10 µM). β-actin was used as a loading control. (B) Western blot analysis showed p-AKT and total AKT as well as β-actin protein bands in confluent HemSCs cultures exposed to the indicated treatments for 1 h. Groups: No treatment; IGF-2 (100 ng/ml); IGF-2 (100 ng/ml) plus OSI-906 (1 µM); IGF-2 (100 ng/ml) plus LY294002 (10 µM). Quantification of the p-AKT protein levels showed an obvious increase in the IGF-2 group (P<0.05). No changes were detected in the total AKT protein levels among the groups (P>0.05). Values are mean ± SD. n =3, *P<0.05 vs. Control group. # P<0.05 vs. IGF-2. IGF-2, insulin-like growth factor-2; HemSC, hemangioma stem cell; p-AKT, phosphorylated AKT.

Article Snippet: The cells were cultured in EGM-2/FBS-10% containing IGF-2 (100 ng/ml), IGF-2 (100 ng/ml) plus OSI-906 (1 μM), IGF-2 (100 ng/ml) plus LY294002 (cat. no. HY-10108; 10 μM; MedChem Express Co., Ltd., Shanghai, China), or no treatment.

Techniques: Expressing, Western Blot, Control

Journal: Experimental and Therapeutic Medicine

Article Title: Insulin-like growth factor 2 promotes the adipogenesis of hemangioma-derived stem cells

doi: 10.3892/etm.2018.7132

Figure Lengend Snippet: Analysis of the adipogenic gene and protein expression of propranolol-treated HemSCs. (A) Western blot analysis results of protein levels in propranolol-treated HemSCs for C/EBPα, C/EBPβ, and PPAR in 0, 50, and 100 µM. (B) Quantitative RT-PCR was performed to analyze the expression of C/EBPα, C/EBPβ, and PPAR. (C) Western blot analysis showed IGF-2 and β-actin protein bands in propranolol-treated HemSCs. Values are mean ± SD. n =3; **P<0.01 vs. Control group. HemSC, hemangioma stem cell; IGF-2, insulin-like growth factor-2.

Article Snippet: The cells were cultured in EGM-2/FBS-10% containing IGF-2 (100 ng/ml), IGF-2 (100 ng/ml) plus OSI-906 (1 μM), IGF-2 (100 ng/ml) plus LY294002 (cat. no. HY-10108; 10 μM; MedChem Express Co., Ltd., Shanghai, China), or no treatment.

Techniques: Expressing, Western Blot, Quantitative RT-PCR, Control

Journal: Parasites & Vectors

Article Title: Preliminary evaluation of the protective effects of recombinant AMA1 and IMP1 against Eimeria stiedae infection in rabbits

doi: 10.1186/s13071-022-05492-4

Figure Lengend Snippet: Trial design and vaccine procedures

Article Snippet: The separated r Es AMA1 and r Es IMP1 were transferred onto nitrocellulose (NC) membranes (Boster, Wuhan, China), separately.

Techniques: Infection, Sterility, Injection

Journal: Parasites & Vectors

Article Title: Preliminary evaluation of the protective effects of recombinant AMA1 and IMP1 against Eimeria stiedae infection in rabbits

doi: 10.1186/s13071-022-05492-4

Figure Lengend Snippet: Multiple sequence alignments of IMP1 and AMA1 from different species. a Multiple alignment of E. stiedae IMP1 with IMP1 proteins from other parasites: Eimeria maxima (UniProt: F4MKA6), Eimeria acervulina (UniProt: U6GHD9), Eimeria tenella (UniProt: F4MKA7), Eimeria mitis (UniProt: U6JVY4), Eimeria praecox (UniProt: U6GBP2); ( b ) multiple alignment of E. stiedae AMA1 with AMA1 proteins from other parasites: Eimeria tenella (UniProt: U6KTA0), Eimeria brunetti (UniProt: U6LBB9), Toxoplasma gondii (UniProt: B6KAM0), Besnoitia besnoiti (UniProt: A0A2A9MBX4), Neospora caninum (UniProt: F0VH85); Blue shading indicates conserved residues. Dashed red boxes represent B-cell epitopes. The transmembrane region is marked with a solid red box

Article Snippet: The separated r Es AMA1 and r Es IMP1 were transferred onto nitrocellulose (NC) membranes (Boster, Wuhan, China), separately.

Techniques: Sequencing

Journal: Parasites & Vectors

Article Title: Preliminary evaluation of the protective effects of recombinant AMA1 and IMP1 against Eimeria stiedae infection in rabbits

doi: 10.1186/s13071-022-05492-4

Figure Lengend Snippet: SDS-PAGE and Western blotting analysis of r Es IMP1 ( a ) and r Es AMA1 ( b ). Lane M: Protein molecular weight markers; lanes 1–4: recombinant proteins that were dissolved in the supernatant, 4 M urea, 6 M urea, and 8 M urea after ultrasonication; lane 5: purified recombinant proteins; lane 6: purified recombinant proteins incubated with anti- E. stiedae positive sera; lane 7: purified recombinant proteins incubated with negative sera from coccidia-free rabbits (the bands are indicated using arrows)

Article Snippet: The separated r Es AMA1 and r Es IMP1 were transferred onto nitrocellulose (NC) membranes (Boster, Wuhan, China), separately.

Techniques: SDS Page, Western Blot, Molecular Weight, Recombinant, Purification, Incubation

Journal: Parasites & Vectors

Article Title: Preliminary evaluation of the protective effects of recombinant AMA1 and IMP1 against Eimeria stiedae infection in rabbits

doi: 10.1186/s13071-022-05492-4

Figure Lengend Snippet: Protective effects of r Es AMA1 and r Es IMP1 against E. stiedae infection under different evaluation indicators

Article Snippet: The separated r Es AMA1 and r Es IMP1 were transferred onto nitrocellulose (NC) membranes (Boster, Wuhan, China), separately.

Techniques: Infection

Journal: Parasites & Vectors

Article Title: Preliminary evaluation of the protective effects of recombinant AMA1 and IMP1 against Eimeria stiedae infection in rabbits

doi: 10.1186/s13071-022-05492-4

Figure Lengend Snippet: Gross postmortem examinations of the liver. a PBS-uninfected group; ( b ) PBS-infected group; ( c ) Quil-A-infected group; ( d ) Trx-His-S-infected group; ( e ) r Es AMA1 group; ( f ) r Es IMP1 group

Article Snippet: The separated r Es AMA1 and r Es IMP1 were transferred onto nitrocellulose (NC) membranes (Boster, Wuhan, China), separately.

Techniques: Infection

Journal: Parasites & Vectors

Article Title: Preliminary evaluation of the protective effects of recombinant AMA1 and IMP1 against Eimeria stiedae infection in rabbits

doi: 10.1186/s13071-022-05492-4

Figure Lengend Snippet: Biochemical estimation of ALT and AST levels in the six groups

Article Snippet: The separated r Es AMA1 and r Es IMP1 were transferred onto nitrocellulose (NC) membranes (Boster, Wuhan, China), separately.

Techniques:

Journal: Parasites & Vectors

Article Title: Preliminary evaluation of the protective effects of recombinant AMA1 and IMP1 against Eimeria stiedae infection in rabbits

doi: 10.1186/s13071-022-05492-4

Figure Lengend Snippet: Changes in serum antibody levels. The changes in serum anti-r Es IMP1 ( a ) and r Es AMA1 ( b ) antibody levels after the first vaccination (week 0, indicated by arrows), booster vaccination (week 2, indicated by arrows), and infection with E. stiedae (week 4, indicated by an arrow)

Article Snippet: The separated r Es AMA1 and r Es IMP1 were transferred onto nitrocellulose (NC) membranes (Boster, Wuhan, China), separately.

Techniques: Infection

Journal: BMC Cancer

Article Title: Insulin-like growth factors in endometrioid adenocarcinoma: Correlation with clinico-pathological features and estrogen receptor expression

doi: 10.1186/1471-2407-12-262

Figure Lengend Snippet: The primers and TaqMan probes of IGF, ER genes used for RT-PCR

Article Snippet: Bio-engineering Co ., Ltd ., Wuhan , China), IGF-2R (polyclonal rabbit anti-human IGF-2R, 1:50 dilution, Wuhan Boster .

Techniques:

Journal: BMC Cancer

Article Title: Insulin-like growth factors in endometrioid adenocarcinoma: Correlation with clinico-pathological features and estrogen receptor expression

doi: 10.1186/1471-2407-12-262

Figure Lengend Snippet: The mRNA levels of IGF-1, IGF-1R, IGF-2, IGF-2R, IGFBP-3, ERα and ERβ in EAC, tumor-adjacent, and control groups

Article Snippet: Bio-engineering Co ., Ltd ., Wuhan , China), IGF-2R (polyclonal rabbit anti-human IGF-2R, 1:50 dilution, Wuhan Boster .

Techniques: Control

Journal: BMC Cancer

Article Title: Insulin-like growth factors in endometrioid adenocarcinoma: Correlation with clinico-pathological features and estrogen receptor expression

doi: 10.1186/1471-2407-12-262

Figure Lengend Snippet: The immunostaining of IGF-1R, IGF-2R, ERα and ERβ in EAC, atypical hyperplasia, and control groups

Article Snippet: Bio-engineering Co ., Ltd ., Wuhan , China), IGF-2R (polyclonal rabbit anti-human IGF-2R, 1:50 dilution, Wuhan Boster .

Techniques: Immunostaining, Control, Positive Control

Journal: BMC Cancer

Article Title: Insulin-like growth factors in endometrioid adenocarcinoma: Correlation with clinico-pathological features and estrogen receptor expression

doi: 10.1186/1471-2407-12-262

Figure Lengend Snippet: The relationship between the IGF and ER mRNA expression and clinico-pathological features of endometrioid adenocarcinoma

Article Snippet: Bio-engineering Co ., Ltd ., Wuhan , China), IGF-2R (polyclonal rabbit anti-human IGF-2R, 1:50 dilution, Wuhan Boster .

Techniques: Expressing

Journal: BMC Cancer

Article Title: Insulin-like growth factors in endometrioid adenocarcinoma: Correlation with clinico-pathological features and estrogen receptor expression

doi: 10.1186/1471-2407-12-262

Figure Lengend Snippet: The relationship between the IGF-1R, IGF2R, ERα and ERβ protein expression and clinico-pathological features of endometrioid adenocarcinoma

Article Snippet: Bio-engineering Co ., Ltd ., Wuhan , China), IGF-2R (polyclonal rabbit anti-human IGF-2R, 1:50 dilution, Wuhan Boster .

Techniques: Expressing

Journal: BMC Cancer

Article Title: Insulin-like growth factors in endometrioid adenocarcinoma: Correlation with clinico-pathological features and estrogen receptor expression

doi: 10.1186/1471-2407-12-262

Figure Lengend Snippet: Correlation analysis of IGF and ER mRNA expression with clinico-pathological features of EAC

Article Snippet: Bio-engineering Co ., Ltd ., Wuhan , China), IGF-2R (polyclonal rabbit anti-human IGF-2R, 1:50 dilution, Wuhan Boster .

Techniques: Expressing

Journal: BMC Cancer

Article Title: Insulin-like growth factors in endometrioid adenocarcinoma: Correlation with clinico-pathological features and estrogen receptor expression

doi: 10.1186/1471-2407-12-262

Figure Lengend Snippet: Correlation analysis of IGF-1R, IGF-2R, and ERα and ERβ protein expression with clinico-pathological features of EAC

Article Snippet: Bio-engineering Co ., Ltd ., Wuhan , China), IGF-2R (polyclonal rabbit anti-human IGF-2R, 1:50 dilution, Wuhan Boster .

Techniques: Expressing

Journal: BMC Cancer

Article Title: Insulin-like growth factors in endometrioid adenocarcinoma: Correlation with clinico-pathological features and estrogen receptor expression

doi: 10.1186/1471-2407-12-262

Figure Lengend Snippet: The analysis of the correlation between the mRNA expression of IGFs with ER subtypes in three groups

Article Snippet: Bio-engineering Co ., Ltd ., Wuhan , China), IGF-2R (polyclonal rabbit anti-human IGF-2R, 1:50 dilution, Wuhan Boster .

Techniques: Expressing, Control